Inclined selective plane illumination microscopy adaptor for conventional microscopes
نویسندگان
چکیده
منابع مشابه
Selective plane illumination microscopy techniques in developmental biology.
Selective plane illumination microscopy (SPIM) and other fluorescence microscopy techniques in which a focused sheet of light serves to illuminate the sample have become increasingly popular in developmental studies. Fluorescence light-sheet microscopy bridges the gap in image quality between fluorescence stereomicroscopy and high-resolution imaging of fixed tissue sections. In addition, high d...
متن کاملDeep imaging in scattering media with selective plane illumination microscopy.
In most biological tissues, light scattering due to small differences in refractive index limits the depth of optical imaging systems. Two-photon microscopy (2PM), which significantly reduces the scattering of the excitation light, has emerged as the most common method to image deep within scattering biological tissue. This technique, however, requires high-power pulsed lasers that are both exp...
متن کاملAdvancing ovarian folliculometry with selective plane illumination microscopy
Determination of ovarian status and follicle monitoring are common methods of diagnosing female infertility. We evaluated the suitability of selective plane illumination microscopy (SPIM) for the study of ovarian follicles. The large field of view and fast acquisition speed of our SPIM system enables rendering of volumetric image stacks from intact whole porcine ovarian follicles, clearly visua...
متن کاملEven fluorescence excitation by multidirectional selective plane illumination microscopy (mSPIM).
Multidirectional selective plane illumination microscopy (mSPIM) reduces absorption and scattering artifacts and provides an evenly illuminated focal plane. mSPIM solves two common problems in light-sheet-based imaging techniques: The shadowing in the excitation path due to absorption in the specimen is eliminated by pivoting the light sheet; the spread of the light sheet by scattering in the s...
متن کاملMiniaturized selective plane illumination microscopy for high-contrast in vivo fluorescence imaging.
Light-sheet-based fluorescence imaging techniques rely on simultaneous excitation of a single optical plane and thus permit high-contrast optically sectioned imaging of extended tissue samples. Here, we introduce a miniaturized fiber-optic implementation of a selective plane-illumination microscope (miniSPIM). The excitation light was delivered through a single-mode optical fiber, and a light-s...
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ژورنال
عنوان ژورنال: Microscopy Research and Technique
سال: 2012
ISSN: 1059-910X
DOI: 10.1002/jemt.22089